Mouse Hematology: A Laboratory Manual

MouseHematology Mike McGarry, Ph.D., our Vice President of Preclinical Studies, along with his Arizona Mayo Clinic colleagues Cheryl A. Protheroe and James J. Lee, has just authored a new book, Mouse Hematology: A Laboratory Manual, now available for pre-order from Cold Spring Harbor Laboratory Press (ships in December):

Dr. McGarry, a preclinical research scientist with over 35 years of experience generating animal-based data, leads preclinical program design, management and execution on behalf of Medelis’ biotech clients around the world.

Here’s the description from CSH Press:  

The mouse has become a standard laboratory model organism, particularly for the study of hematopoiesis, the immune system, and inflammation. Although laboratories studying stem cells, blood, and blood-forming tissues have assimilated many new molecular diagnostic methods, the identification of cell lineages through classical light microscopic techniques is often poorly understood and practiced. Mouse Hematology presents a concise review of conventional methods for the preparation, enumeration, and microscopic examination of blood and blood-forming tissues of the laboratory mouse. Along with a short laboratory manual featuring detailed protocols, Mouse Hematology includes a DVD of short video demonstrations of the techniques and a poster of blood cell types for easy identification at the microscope. These rapid, inexpensive assessments can save valuable time and resources essential to the design, development, and interpretation of experiments.     

Contents include:

  1. Collection of Peripheral Blood
  2. Counting Red Blood Cells, Platelets, and Viable Nucleated White Blood Cells
  3. Peripheral Blood Films and Cytospin Preparations
  4. Cell Differential Assessments of Peripheral Blood Films
  5. Preparation of Bone Marrow for Microscopic Examination
  6. Cell Differential Assessments of Bone Marrow

The book also includes 8 videos on an accompanying DVD:

  1. Venous Access for Blood Film Using Lateral Tail Vein
  2. Venous Access for Blood Film Using Tip of Tail
  3. Blood Film
  4. Staining with Coplin Jar or Carriages
  5. Coverslip
  6. Cytocentrifuge Procedures
  7. Surgical Exposure of Femur
  8. Brush Smear of Marrow

To preorder, visit the CSH Press website: